Detection of p53 gene mutation in peripheral blood in the diagnosis of patients with lung cancer / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>To study the diagnostic significance of detecting p53 gene mutation in peripheral blood of lung cancer patients.</p><p><b>METHODS</b>PCR-SSCP was used to detect p53 gene mutation in peripheral blood of 46 samples (lung cancer 28, benign lung diseases 8, healthy people 10).</p><p><b>RESULTS</b>Of the 28 lung cancer patients, 4 cases (squamous cell carcinoma 1, adenocarcinoma 1, small cell lung cancer 2) had p53 gene mutation. The positive rate was 14.29%. Of the 4 positive lung cancer patients, one patient was in stage II, one in stage III, and the other two in stage IV. No p53 mutation was found in peripheral blood of 8 benign lung disease patients and 10 healthy persons.</p><p><b>CONCLUSIONS</b>Detection of p53 gene mutation in peripheral blood cells may be helpful to the diagnosis of lung cancer, especially to the accurate staging of lung cancer.</p>