Genotyping of ABO loci in para-Bombay type individuals / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 513-516, 2004.
Artículo
en Chino
| WPRIM
| ID: wpr-352030
ABSTRACT
To study the molecular genetic basis of ABO alleles in para-Bombay type individuals, samples from five para-Bombay type individuals identified by serologic tests including absorption-elution tests, saliva neutralizing or inhibitor substances tests, were genotyped by using PCR-SSP based ABO genotyping. Exon 6 and exon 7 at the ABO locus for all 5 samples were sequenced. The results showed that the ABO genotypes of five para-Bombay samples were A102B1, A102B1, A102O1, A102B1, B1O1 respectively, the direct DNA sequencing results were in accordance with the results genotyped by PCR-SSP method, No novel nucleotide mutation was found at the exon 6 and exon 7 of ABO gene. In conclusion, the ABO genotyping assay by PCR-SSP provide a simple, rapid and accurate method for determining the ABO type of para-Bombay cases.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Sistema del Grupo Sanguíneo ABO
/
Reacción en Cadena de la Polimerasa
/
Mapeo Cromosómico
/
Análisis de Secuencia de ADN
/
Alelos
/
Genética
/
Genotipo
Tipo de estudio:
Estudio pronóstico
Límite:
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2004
Tipo del documento:
Artículo
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