Detection of cytokine-secreting T lymphocytes in allogeneic reaction and a preliminary study on its clinical significance / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 498-502, 2004.
Article
en En
| WPRIM
| ID: wpr-352033
Biblioteca responsable:
WPRO
ABSTRACT
In order to explore a new way to study allogeneic reactive T lymphocytes, detection of cytokine-secreting T lymphocytes after allogeneic peripheral blood mononuclear cells (PBMNCs) stimulation and investigation of its clinical significance were performed. A novel cytokine secretion assay (CKSA) was first applied to detect T lymphocytes secreting cytokine including IFN-gamma, IL-4 and IL-10 at single cell level in human mixed lymphocytes reaction. IFN-gamma-secreting T cells from PBMNCs were then evaluated in 2 patients with acute graft versus host disease (aGVHD) after allogeneic bone marrow transplantation. The results showed that compared with IL-4 and IL-10 (which were 0.12 +/- 0.03% and 0.10 +/- 0.03% respectively), a sizable proportion of IFN-gamma-secreting T lymphocytes could be detected (1.12 +/- 0.13)% after allogeneic PBMNCs stimulation. Preliminary results indicated that frequency of IFN-gamma-secreting T lymphocytes correlated with the onset and severity of clinical aGVHD. In conclusion, it is feasible to detect IFN-gamma secreting T lymphocytes after allogeneic PBMNCs stimulation and to apply the CKSA technique for clinical identification of aGVHD.
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WPRIM
Asunto principal:
Trasplante Homólogo
/
Linfocitos T
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Enfermedad Aguda
/
Citocinas
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Interleucina-4
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Interferón gamma
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Interleucina-10
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Secreciones Corporales
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Alergia e Inmunología
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Enfermedad Injerto contra Huésped
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Journal of Experimental Hematology
Año:
2004
Tipo del documento:
Article