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A simple and rapid method for propagation and purification of the peripheral blood gammadeltaT cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 372-374, 2004.
Artículo en Inglés | WPRIM | ID: wpr-352062
ABSTRACT
The purpose of this study was to set up an approach for expansion of the peripheral blood gammadeltaT cells from normal subjects in order to explore the characteristics of gammadeltaT cells. Peripheral blood mononuclear cells (PBMNC) were separated from 5 - 10 ml peripheral blood and stimulated by the low molecular peptide derived from Mycobacterium tuberculosis (MTb-Ag), and expanded in rIL-2-containing medium. The relative amount of gammadeltaT cells were measured by anti TCR gammadelta-PE staining and flow cytometry. The Cytotoxicity were detected by gammadeltaT assay. The results showed that after stimulation and expansion for 10 days, gammadeltaT cells increased to 69.2% of the total PBMNC and demonstrated significant cytotoxicity against K562 cells. In conclusion, this is a simple, rapid and specific method for expansion of peripheral blood gammadeltaT cells in vitro.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Separación Celular / Subgrupos de Linfocitos T / Receptores de Antígenos de Linfocitos T gamma-delta / Biología Celular / Alergia e Inmunología / Citometría de Flujo / Métodos / Mycobacterium tuberculosis / Antígenos Bacterianos Límite: Humanos Idioma: Inglés Revista: Journal of Experimental Hematology Año: 2004 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Separación Celular / Subgrupos de Linfocitos T / Receptores de Antígenos de Linfocitos T gamma-delta / Biología Celular / Alergia e Inmunología / Citometría de Flujo / Métodos / Mycobacterium tuberculosis / Antígenos Bacterianos Límite: Humanos Idioma: Inglés Revista: Journal of Experimental Hematology Año: 2004 Tipo del documento: Artículo