An experimental study of inhibiting effect of decoy oligodeoxynucleotides on the gene expression of collagen in NIH3T3 cell / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 175-178, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-352291
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of activator protein-1 (AP-1) decoy-oligodeoxynucleotides (Decoy-ODNs) on the expression of fibroblast alpha2 type I collagen, so as to explore the gene therapy of pathologic scar.</p><p><b>METHODS</b>Decoy-ODNs targeting AP-1 were designed and synthesized. NIH3T3 cells were transfected by cationic liposomes. The distribution of Decoy-ODNs in the cells was investigated. The inhibiting effects of Decoy-ODNs on AP-1 were determined by electrophoretic mobility shift assay (EMSA). And the effects of Decoy-ODNs on the collagen synthesis in the cells were analyzed by RT-PCR.</p><p><b>RESULTS</b>AP-1 Decoy-ODNs could competitively inhibit the AP-1 in vitro activity. Cationic liposomes could play roles by effectively transfecting Decoy-ODNs into the plasma and nucleus. The mRNA expression of fibroblast alpha2 type I collagen decreased evidently after 24 hours of Decoy-ODNs action.</p><p><b>CONCLUSION</b>Decoy-ODNs could inhibit the mRNA expression of fibroblast alpha2 type I collagen by antagonizing AP-1.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Oligodesoxirribonucleótidos
/
Farmacología
/
ARN Mensajero
/
Transfección
/
Factor de Transcripción AP-1
/
Colágeno Tipo I
/
Células 3T3 NIH
/
Fibroblastos
/
Genética
/
Metabolismo
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Burns
Año:
2003
Tipo del documento:
Artículo
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