The proteomics research on relational expressed serum proteins among the recovered SARS patients complicating avascular necrosis of femoral head / 中华预防医学杂志
Chinese Journal of Preventive Medicine
; (12): 522-526, 2008.
Article
en Zh
| WPRIM
| ID: wpr-352451
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To seek differentially expressed serum proteins in recovered SARS patients complicating avascular necrosis of femoral head (AVNFH).</p><p><b>METHODS</b>2-DE and MALDI-TOF MS were used to study the comparative serum proteomics among female SARS AVNFH group, female SARS non-AVNFH group and female healthy group. ELISA method was used to detect serum amyloid P component in individual serum; specificity and sensitivity of serum amyloid P component were analyzed.</p><p><b>RESULTS</b>Average protein points on 2-DE of 3 groups were 632 +/- 28, 671 +/- 55, 688 +/- 42 respectively, and the matching rate of protein points was ranged from 85% to 95%; eighteen differentially expressed proteins were discovered including transthyretin, serpin peptidase inhibitor, alpha-1-antitrypsin precursor, serum amyloid P components, etc. Compared to healthy group and SARS non-AVNFH group, transthyretin, C4B3, fibrinogen gamma, apolipoprotein L, apolipoprotein A-IV precursor, albumin and prealbumin showed lower expression, inversely serpin peptidase inhibitor, alpha-1-antitrypsin precursor and serum amyloid P components showed higher expression in serum in the SARS AVNFH necrosis group. The serum amyloid P component in 3 groups were 0.54 +/- 0.30 ng/ml, 0.83 +/- 0.39 ng/ml, 1.21 +/- 0.29 ng/ml respectively. The areas under the ROC curve on serum amyloid P component was 0.854, the specificity was 77.8% and the sensitivity was 85.2%.</p><p><b>CONCLUSION</b>There were differentially expressed serum proteins in three groups. Serum amyloid P components might be one of the potential biomarkers in serum of recovered SARS patients complicating avascular necrosis of femoral head.</p>
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Índice:
WPRIM
Asunto principal:
Sangre
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Proteínas Sanguíneas
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Electroforesis en Gel Bidimensional
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Estudios de Casos y Controles
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Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
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Síndrome Respiratorio Agudo Grave
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Proteómica
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Necrosis de la Cabeza Femoral
Tipo de estudio:
Observational_studies
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Prognostic_studies
Límite:
Adult
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Female
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Humans
Idioma:
Zh
Revista:
Chinese Journal of Preventive Medicine
Año:
2008
Tipo del documento:
Article