Expression of Neurofilament Protein in the Nerve of Human Cornea

ABSTRACT

This study was performed to identify the expression of neurofilament in sensory nerve of human cornea. The three normal corneal tissues were dissected. Monoclonal antibody to neurofilament protein were used to visualize the corneal nerve. The one normal cornea was examined to observe the corneal nerve with electron rnicroscope. Transmission electron rnicroscope showed that the corneal nerve contained the neurofilament within the axon. The indirect iinmunofluorescent techniques revealed the expression of neurofilament in corneal stroma. The results of this study show that immunofluorescent technique may useful method in identification of sensory nerve of human cornea, and make it easy for us to visualize corneal innervation pattern in flat section by using of antibody to neurofilament protein.