Your browser doesn't support javascript.
loading
Exploring the optimal operation time for patients with hypertensive intracerebral hemorrhage: tracking the expression and progress of cell apoptosis of prehematomal brain tissues / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 1246-1250, 2010.
Artículo en Inglés | WPRIM | ID: wpr-352581
ABSTRACT
<p><b>BACKGROUND</b>Hypertensive intracerebral hemorrhage (HICH) is a severe disease with high morbidity and mortality. Timely removal of the hematoma through surgical procedures may effectively reduce secondary injuries. However, there has long been a debate over the proper timing of such surgery. In this study, we explored the optimal operation time for HICH patients by observing the pathological changes in perihematomal brain regions during different stages of onset.</p><p><b>METHODS</b>Twenty-five specimens of brain tissue, obtained from perihematomal region of HICH patients in different phases, were subjected to haematoxylin-eosin (HE) staining, terminal deoxynucleotidyl transferase-mediated deoxyuridine 5-triphosphate nick-end labeling (TUNEL) staining and Caspase-3, matrix metalloproteinases-9 (MMP-9) immunohistochemical staining. The changing roles of necrosis and apoptosis and the expression of MMP-9 and Caspase-3 positive cells were all observed using image analysis.</p><p><b>RESULTS</b>The obvious expression of TUNEL positive cells was recognized within 6 hours of ICH onset, reaching its peak between 6 hours and 24 hours in the early phase.</p><p><b>RESULTS</b>were highly consistent with Caspase-3 and MMP-9 positive cell counts. Necrosis was found 6 hours after ICH onset and aggravated after 12 hours.</p><p><b>CONCLUSIONS</b>In the early phase, apoptosis was seen as a major modality of injury in the brain tissue of the perihematomal region and was strongly correlated with the expression of MMP-9 and Caspase-3. The results of the present study suggest that an operation performed as soon as possible after ICH onset may be optimal for preserving the nervous system function.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Cirugía General / Factores de Tiempo / Encéfalo / Apoptosis / Etiquetado Corte-Fin in Situ / Hemorragia Intracraneal Hipertensiva / Metaloproteinasa 9 de la Matriz / Caspasa 3 Límite: Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2010 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Patología / Fisiología / Cirugía General / Factores de Tiempo / Encéfalo / Apoptosis / Etiquetado Corte-Fin in Situ / Hemorragia Intracraneal Hipertensiva / Metaloproteinasa 9 de la Matriz / Caspasa 3 Límite: Anciano / Femenino / Humanos / Masculino Idioma: Inglés Revista: Chinese Medical Journal Año: 2010 Tipo del documento: Artículo