Volume-activated Cl- current in migrated nasopharyngeal carcinoma cells / 生理学报
Acta Physiologica Sinica
;
(6): 525-530, 2004.
Artículo
en Inglés
| WPRIM
| ID: wpr-352739
ABSTRACT
The transwell chamber migration assay and the patch-clamp technique were used to investigate the volume-activated Cl(-) current (I(Cl.vol)) in migrated nasopharyngeal carcinoma cells (CNE-2Z). 47% hypotonic solution activated a ICl.vol in the migrated CNE-2Z cells. Compared with the control cells (non-migrated), the properties of this current and the sensitivity to Cl(-) channel blockers were changed. The current density in migrated CNE-2Z cells was higher than that in non-migrated cells. The current was almost completely inhibited by extracellular application of adenosine-5'-triphosphate (ATP, 10 mmol/L), 5-nitro-2-3-phenylpropylamino benzoic acid (NPPB, 100 mmol/L) and tamoxifen (30 mmol/L) in all voltage steps applied. The inhibition of NPPB and tamoxifen on the current was stronger in migrated cells than that in non-migrated cells. The permeability sequence of the four anions was Br(-)>Cl(-)> I (-)>Gluconate. The sequence was different from that of the non-migrated cells (I(-)> Br(-)> Cl(-)> Gluconate). The results suggest that volume-activated chloride channels may be involved in the CNE-2Z cell migration.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Fisiología
/
Tamoxifeno
/
Células Tumorales Cultivadas
/
Carcinoma
/
Ciclo Celular
/
División Celular
/
Movimiento Celular
/
Cloruros
Límite:
Humanos
Idioma:
Inglés
Revista:
Acta Physiologica Sinica
Año:
2004
Tipo del documento:
Artículo
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