Establishment of computer-based video-tracking conditioned place preference experiment system in mice / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To establish the computer-based video-tracking conditioned place preference (CPP) system in mice.</p><p><b>METHODS</b>The CPP system was composed of computer, camera, soundproof box, shuttle box and analytical software. The results of morphine-induced conditioned place preference were used to evaluate the experiment system. And the effect of morphine-induced locomotor activity in drug-paired compartment was studied in mice.</p><p><b>RESULTS</b>Low (1 mg/kg, i.p.), moderate (3 mg/kg, 5 mg/kg, i.p.) and high (10 mg/kg, i.p.) dose of morphine significantly prolonged the time mice spent in drug-paired compartment compared with saline, but there was no dose-response relation. Moderate and high dose of morphine significantly enhanced locomotor activity, among which 5 mg/kg and 10 mg/kg morphine induced behavior sensitization in drug-paired compartment during the conditioning sessions.</p><p><b>CONCLUSION</b>The computer-based video-tracking conditioned place preference experiment system in mice established successfully is reliable and stable.</p>