Apoptotic mechanism of WJH-6, a novel polyamine conjugate, on K562 and HL-60 cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 451-455, 2010.
Article
en Zh
| WPRIM
| ID: wpr-353377
Biblioteca responsable:
WPRO
ABSTRACT
In the present study, the apoptotic mechanism and polyamine transporter recognition of WJH-6, a novel polyamine conjugate, were investigated in K562 and HL-60 cells. The cytotoxicity of WJH-6 was assessed by MTT assay; cell cycle distribution and apoptosis were measured by flow cytometry; the protein expression of Caspase-3, Caspase-8, Caspase-9, Bid and mitochondrial membrane potential (MMP) were evaluated by high content screening (HCS) analysis; the protein expression of cytochrome c was measured by Western blotting. The results showed that WJH-6 could be recognized and transported by polyamine transporter (PAT). Furthermore, WJH-6 was able to inhibit K562 and HL-60 cells proliferation and induce apoptosis. This apoptotic effect was relative to MMP loss, cytochrome c release from mitochondria to cytoplasm and the activation of Caspase-8, Caspase-9, Caspase-3 and Bid. These results suggested that WJH-6-induced K562 and HL-60 cells apoptosis was related with mitochondrial damage.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Farmacología
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Poliaminas
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Ciclo Celular
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Apoptosis
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Células HL-60
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Células K562
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Citoplasma
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Citocromos c
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Proliferación Celular
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Activación Enzimática
Límite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Año:
2010
Tipo del documento:
Article