Molecular identification of medicinal plants: Dendrobium chrysanthum, Dendrobium fimbriatum and their morphologically allied species by PCR-RFLP analyses / 药学学报
Acta Pharmaceutica Sinica
;
(12): 728-733, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-353420
ABSTRACT
<p><b>AIM</b>To establish a simple method for molecular identification of original plants of D. chrysanthum and D. fimbriatum using molecular marker rDNA ITS region.</p><p><b>METHODS</b>Restriction patterns of ITS fragments were obtained using PCR-RFLP method. The PCR products of D. chrysanthum and its morphologically allied species were digested at 37 degrees C by Cla I and Apa LI, those of D. fimbriatum and its morphologically allied species were digested by Sph I.</p><p><b>RESULTS</b>D. chrysanthum, D. fimbriatum and their morphologically allied species could be identified by predicted restriction profiles of PCR-RFLP. The botanical origin of twenty-five fresh samples of "Shihu" collected in markets was identified by this method.</p><p><b>CONCLUSION</b>The results showed that PCR-RFLP analysis of the rDNA ITS region is a feasible, simple and inexpensive method for determining the botanical origin of the traditional Chinese medicine "Shihu".</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Plantas Medicinales
/
Especificidad de la Especie
/
Polimorfismo de Longitud del Fragmento de Restricción
/
ADN Ribosómico
/
Reacción en Cadena de la Polimerasa
/
Contaminación de Medicamentos
/
Clasificación
/
Análisis de Secuencia de ADN
/
ADN de Plantas
/
Dendrobium
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Acta Pharmaceutica Sinica
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS