Effect of phytohemagglutinin (PHA) from Yunnan white kidney bean on development of mouse embryos / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1665-1669, 2011.
Article
en Zh
| WPRIM
| ID: wpr-354180
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of different concentration of phytohemagglutinin (PHA) on mouse embryo development.</p><p><b>METHOD</b>In experiment 1, crude and purified PHA extracted from Yunnan white kidney bean with different concentration were added into M16 culture medium, the final concentration of PHA were: 50, 100, 200, 500, 1 000, 2 000 and 5 000 mg x L(-1) respectively. 2-cell stage embryos were collected and cultured in PHA containing or control medium for 72-96 h and their development were recorded. In experiment 2, different stage of embryos from 1-cell to blastocyst were treated by different concentrations of PHA same as experiment 1 and 10 000 mg x L(-1) in culture medium for 24 h before washing and cultured in M16 + PVA without PHA to blastocyst or hatching blastocyst stage.</p><p><b>RESULT</b>Low concentrations PHA at 50-100 mg x L(-1) promoted embryo development and increased the number of blastocyst stage embryos. In contrast, high concentrations of PHA (> 1 000 mg x L(-1)) blocked the embryos development from 1-cell to blastocyst stage and showed apoptosis morphology or death.</p><p><b>CONCLUSION</b>Depending on the concentrations, PHA from white kidney bean shown promotion or inhibition on mouse embryo development. 1-cell stage embryo shown more sensitive to PHA treatment than that of later stage embryos. Pretreatment 24 h in PHA containing medium can influence the further development of embryos. Low concentrations of PHA is benefit to embryo development, but high concentrations of PHA (> 1 000 mg x L(-1)) will block of the development of embryos.</p>
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Índice:
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Asunto principal:
Farmacología
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Fitohemaglutininas
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Química
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Phaseolus
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Desarrollo Embrionario
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Embrión de Mamíferos
Límite:
Animals
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Pregnancy
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Article