Compatibility of a novel ethylenediamine modified polylactic acid with osteoblasts / 生物医学工程学杂志

ABSTRACT

Biocompatibility of a newly developed ethylenediamine modified poly (DL-latic acid) (EMPLA) with osteoblasts was investigated by means of cell morphology and cell proliferation. Films of PLA and EMPLA were made by solvent casting. Osteoblasts obtained from crania of neonatal Wistar rats were cultured on surfaces of PLA and EMPLA, with glass as control. The cell morphology was observed by phase contrast microscope and the cell proliferation was determined by MTT assay. The morphology observations revealed that the osteoblasts cultured on EMPLA spread wider than those on PLA, and much more cells were confluent on EMPLA, compared to those on PLA and glass. The growth curves showed the osteoblasts on EMPLA grew faster than did those on PLA and glass. The results exhibited that the biocompatibility of EMPLA with osteoblasts is better than that of PLA and glass, which suggested wide applications of EMPLA in biomedical area, especially in tissue engineering.