Primary research of reconstruction of complete biological tissue engineered blood vessel / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 448-451, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-354277
ABSTRACT
To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells (VSMCs) were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Arterias
/
Porcinos
/
Prótesis Vascular
/
Células Cultivadas
/
Técnicas de Cocultivo
/
Biología Celular
/
Ingeniería de Tejidos
/
Miocitos del Músculo Liso
/
Microscopía Electrónica de Transmisión
/
Andamios del Tejido
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS