Establishing a high-titer infectious avian influenza A (H5N1) pseudotyped viral particle / 病毒学报
Chinese Journal of Virology
;
(6): 324-329, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-354728
ABSTRACT
A transient four-plasmid cotransfection system was used to construct avian influenza A (H5N1) pseudotyped viral particle (H5N1Pp) by incorporating hemagglutinin (HA) protein and neuraminidase (NA) protein from H5N1 avian influenza virus onto Murine leukemia virus pseudotyped viral particles, the transmission electron microscopy, infectivity titer assay, hemagglutination assay, neutralization assay of H5N1Pp were studied. We established a pseudotyped H5N1 viral particle at a high titer of 10(8) Pp/mL, the morphology,the hemagglutination activity and neutralization specificity of H5N1Pp is simililar to wild H5N1 virus. The research result sets a platform for studying this virus, including its receptors, the functional analysis of HA and NA, neutralizing antibodies and anti-H5N1 drug development.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Virión
/
Virología
/
Aves
/
Pruebas de Neutralización
/
Transfección
/
Ingeniería Genética
/
Glicoproteínas Hemaglutininas del Virus de la Influenza
/
Carga Viral
/
Subtipo H5N1 del Virus de la Influenza A
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2012
Tipo del documento:
Artículo
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