Correlation between TCID50 and p27 antigen of avian leukosis virus subgroup J / 病毒学报
Chinese Journal of Virology
;
(6): 521-525, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-354796
ABSTRACT
To study the correlation between 50% tissue-culture infective dose (TCID50) value and p27 antigen S/P value of Avian leukosis virus subgroup J and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with Avian leukosis virus subgroup J strain NX0101 and samples were tested continuously for ten days after changing maintenance media. The correlation between TCID50 and p27 antigen S/P value of ten days were then analysized. Simultaneously, DF-1 cells were inoculated with NX0101 and passaged to 20 generations. Samples taken from 1st generation, 5th generation, 10th generation, 15th generation and 20th generation were tested for the TCID50 titer and the p27 antigen S/P value separately. A significant Pearson correlation was found between them in CEF cells (r = 0.85277; P < 0.0001) and in DF-1 cells (r = 0.93000; P = 0.0220). This study provided an important parameter for predicting TCID50 by detecting the p27 antigen S/P value.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Virología
/
Virulencia
/
Leucosis Aviar
/
Virus de la Leucosis Aviar
/
Antígeno Nuclear de Célula en Proliferación
/
Carga Viral
/
Alergia e Inmunología
/
Fibroblastos
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2011
Tipo del documento:
Artículo
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