Expression of structural and non-structural proteins of severe fever with thrombocytopenia syndrome bunyavirus / 病毒学报
Chinese Journal of Virology
; (6): 515-520, 2011.
Article
en Zh
| WPRIM
| ID: wpr-354797
Biblioteca responsable:
WPRO
ABSTRACT
Severe fever with thrombocytopenia syndrome bunyavirus (SFTSV) is a novel phlebovirus, causing a life-threatening illness associated with the symptoms of severe fever and thrombocytopenia syndrome. The sequence and structure of the genome have already been illustrated in previous study. However, the characteristics and function of the structure and non-structure proteins is still unclear. In this study, we identified the density of the purified SFTSV virions as 1.135 g/mL in sucrose solution. Using RT-PCR method, we amplified the full coding sequence of RNA dependent RNA polymerase(RdRp), glycoprotein precursor (M), glycoprotein n (Gn), glycoprotein c (Gc), nuclear protein (NP) and non structural protein (NSs) of SFTSV (strain HB29). Respectively inserted the target genes into eukaryotic expression vector pcDNA5/FRT or VR1012, the target protein in 293T cell were successfully expressed. By analyzing the SFTSV virions in SDS-PAGE and using recombinant viral proteins with SFTS patients sera in Western blotting and Immunofluorescent assay, the molecule weight of structure and non-structure proteins of SFTSV were defined. The study provides the first step to understand the molecular characteristics of SFTSV.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Trombocitopenia
/
Virión
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Virología
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Línea Celular Transformada
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Proteínas Estructurales Virales
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Orthobunyavirus
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Infecciones por Bunyaviridae
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Proteínas no Estructurales Virales
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Células HEK293
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Fiebre
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2011
Tipo del documento:
Article