Experimental study of the specific immunoresponse induced by p53 modified dendritic cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 632-635, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-354813
ABSTRACT
<p><b>OBJECTIVE</b>To explore the specific cytotoxic T lymphocyte (CTL) induced by dendritic cells (DC), which were transfected by the plasmid pC53-SN3 encoding p53 gene.</p><p><b>METHODS</b>DC derived from HLA-A2(+) mononuclear cells of the 24-lung cancer patients was transfected with the plasmid pC53-SN3 by lipofectamine and then co-cultured with auto-unpurified T cells to induce potent CTL (T-pC53-SN3). The cytolysis of specific CTL against Calu-6, a HLA-A2(+) human lung cancer cell line, was measured by using lactate dehydrogenase (LDH) releasing assay.</p><p><b>RESULTS</b>The expression of CD(1a) and CD(83), the correlative markers of DC, increased apparently after transfected with plasmid pC53-SN3, the expression rate was (5.45 +/- 0.89)% and (3.26 +/- 0.47)% versus (52.15 +/- 11.56)% and (25.78 +/- 12.35)%. CD(14) decreased apparently, but other DC correlative markers of CD(1a), CD(40), CD(86), and HLA-DR remained almost the same as that before transfection. Compared with T-IL-2, the CTL derived from PBMNC stimulated by IL-2 (100 U/ml), the cytolytic activity of T-pC53-SN3 against Calu-6 cell line showed a significant increase, but cytolytic activity was 56.79 +/- 15.67 and 39.33 +/- 9.88, respectively, when effect cells target cells was 101. The expression of the CD(8), CD(69), and CD(45)RO/CD(8) of T-pC53-SN3 cells increased significantly, but that of CD(3), CD(4), CD(86), ect, was not significantly different from those of T-pCMV-neo.</p><p><b>CONCLUSIONS</b>It showed that DC transfected by p53 gene could induce potent HLA-A(2) restrictive CTL to kill tumor cell efficiently.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Fisiología
/
Células Dendríticas
/
Glicoproteínas de Membrana
/
Linfocitos T
/
Antígenos CD
/
Factor Estimulante de Colonias de Granulocitos y Macrófagos
/
Proteína p53 Supresora de Tumor
/
Interleucina-4
/
Técnicas de Cocultivo
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Hematology
Año:
2003
Tipo del documento:
Artículo
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