Determination of the repair half-time of human nasopharyngeal carcinoma cell lines CNE1, CNE2, HONE1 and C666-1 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 777-778, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-355020
ABSTRACT
<p><b>OBJECTIVE</b>To determine the repair half-time (T1/2), a speed parameter of sub-lethal damage repair (SLDR), of human nasopharyngeal carcinoma (NPC) cell lines CNE1, HONE1, C666-1 and CNE2.</p><p><b>METHODS</b>A total radiation dose of 8 Gy divided into 4+4 Gy was delivered to the cell lines at the interval of 0 s, 15 s, 30 s, 1 h, 2 h, 4 h or 6 h. The cell survival fractions were determined using the standard cell clonogenic assay. The curves of the changes in the surviving cell fraction after a total dose of 8 Gy, as a function of the interval between the two doses of 4 Gy, were fitted manually, and the T1/2 of each cell line was calculated according to the curves.</p><p><b>RESULTS</b>The T1/2 of CNE1, HONE1, C666-1 and CNE2 were 18 s, 22 s, 29 s and 27 s, respectively.</p><p><b>CONCLUSION</b>The speed of SLDR of NPC cells is quite rapid, indicating that the fraction delivery time longer than 20 s might decrease the effect of radiotherapy.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Efectos de la Radiación
/
Traumatismos por Radiación
/
Radioterapia
/
Supervivencia Celular
/
Neoplasias Nasofaríngeas
/
Radioterapia Conformacional
/
Línea Celular Tumoral
/
Relación Dosis-Respuesta en la Radiación
/
Radioterapia de Intensidad Modulada
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Artículo
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