Tumor-targeted human telomerase reverse transcriptase promoter/tk gene therapy against human nasopharyngeal carcinoma cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 695-699, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-355041
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the targeted killing effect of human telomerase reverse transcriptase promoter (hTERTp)/tk gene on human nasopharyngeal carcinoma (NPC) cells.</p><p><b>METHODS</b>The recombinant plasmid hTERTp/tk/pGL3 was transfected into human NPC HNE1 cells and the expressions of TK and telomerase were investigated. The targeted killing effect induced by hTERTp/tk on HNE1 cells was assessed using RT-PCR and MTT assay.</p><p><b>RESULTS</b>TK gene expression was detected in HNE1 cells transfected by hTERTp/tk/pGL3, and the cells showed reduced telomerase and hTERT expression as compared with the control cells. hTERTp/tk/pGL3 resulted in target killing of HNE1 cells but not of the normal control cells. The tumor cell-killing effect of hTERTp/tk/pGL3 was slightly milder than that of the positive control CMV/tk/pGL3 that produced nonselective cell killing.</p><p><b>CONCLUSION</b>hTERTp/tk, a tumor-specific expression system, allows targeted tumor cell killing and reduces the activity of telomerase in NPC cells in vitro.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Timidina Quinasa
/
Transfección
/
Terapia Genética
/
Neoplasias Nasofaríngeas
/
Regiones Promotoras Genéticas
/
Marcación de Gen
/
Telomerasa
/
Línea Celular Tumoral
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Artículo
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