High glucose up-regulates GRK2 gene expression in H9C2 cardiomyoblasts in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 472-474, 2010.
Article
en Zh
| WPRIM
| ID: wpr-355098
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of high glucose on GRK2 gene expression in H9C2 cardiomyoblasts in vitro.</p><p><b>METHODS</b>H9C2 cardiomyoblasts were cultured for 72 h in the presence of 0, 5.5, 12.5, 25 or 33 mmol/L glucose (with the osmotic pressure adjusted with monnitol). Semi-quantitative detection of GRK2 gene expression in H9C2 cardiomyoblasts was carried out using RT-PCR and phosph-Akt (Ser473) protein level was measured by Western blotting.</p><p><b>RESULTS</b>Glucose in the culture medium (5.5 to 33 mmol/L) concentration-dependently increased the mRNA expression of GRK2 concentration and decreased phosphorylation Akt (ser473) level in in H9C2 cardiomyoblasts.</p><p><b>CONCLUSION</b>Increased GRK2 gene expression may play an important role in cardiac dysfunction in diabetes.</p>
Texto completo:
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Índice:
WPRIM
Asunto principal:
Farmacología
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ARN Mensajero
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Regulación hacia Arriba
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Línea Celular
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Regulación de la Expresión Génica
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Biología Celular
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Miocitos Cardíacos
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Complicaciones de la Diabetes
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Quinasa 2 del Receptor Acoplado a Proteína-G
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Genética
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2010
Tipo del documento:
Article