Purification and anti-cancer activity of ricin / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 217-219, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-355238
ABSTRACT
<p><b>OBJECTIVE</b>To extract and purify ricin from castor beans and to evaluate its anti-cancer activity.</p><p><b>METHODS</b>Ricin was purified from castor beans according the modified method of Nicolson and Blaustin. The lectins were extracted in 0.01 mol/L phosphate buffered saline and isolated in the 40% to 80% fraction of ammonium sulfate precipitation. The dialyzed fractionated preparation was applied with a Sepharose 4B column. The lectins were eluted with a linear lactose gradient (0.01 mol/L approximately 0.5 mol/L). Ricin was separated from the ricinus agglutinin by gel filtration on a Sephadex G-100. MTT was applied to analyze the cytotoxicity with different dosage of ricin in different cancer cell lines.</p><p><b>RESULTS</b>There was no difference between the killing effect of normal cells and that of colon cancer cells by using the high dosage of ricin (5 x 10(-8) mol/L approximately 5 x 10(-10) mol/L). However, the cytotoxicity was significant different in those cells with the low dosage of ricin (5 x 10(-11) mol/L approximately 5 x 10(-13) mol/L). Meanwhile ricin had the similar cytotoxicity to leukemia cell K562 and colon cancer cell SW480.</p><p><b>CONCLUSION</b>Ricin is able to kill tumor cells selectively at low concentration, but the selectivity does not appear at high concentrations.</p>
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Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Farmacología
/
Ricina
/
Linfocitos T Citotóxicos
/
Neoplasias Colorrectales
/
Ratas Sprague-Dawley
/
Células K562
/
Línea Celular Tumoral
/
Relación Dosis-Respuesta a Droga
/
Antineoplásicos Fitogénicos
Límite:
Animales
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Zhejiang University. Medical sciences
Año:
2005
Tipo del documento:
Artículo
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