β-arrestin1 promotes chronic myeloid leukemia cell proliferation by activating JNK signaling pathway / 南方医科大学学报
Journal of Southern Medical University
;
(12): 677-681, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-355305
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the signaling pathways involved in β-arrestin1-induced proliferation of K562 cells.</p><p><b>METHODS</b>We established stable cell lines K562-siβ1 and K562-β1 by lentivirus-mediated β-arrestin1 knock-down or overexpression in K562 cells, with cells transfected with non-specific siRNA as the control (K562-Ctrl). The proliferation of these cells were evaluated by cell counting and CCK-8 assays. Western blotting was used to detect the expression of JNK and p-JNK in the cells, and co-immunoprecipitation (Co-IP) assay was employed to investigate the interaction between β-arrestin1 and Src.</p><p><b>RESULTS</b>K562-β1 cells showed significantly greater but K562-siβ1 cells had significantly lower proliferation ability and cell survival rate than K562-Ctrl cells. Western blotting showed that β-arrestin1 specifically enhanced the expression of p-JNK, and the JNK inhibitor SP600125 obviously suppressed p-JNK and cell proliferation of K562 cells. Co-IP assay revealed the binding of β-arrestin1 to Src.</p><p><b>CONCLUSIONS</b>In K562 cells, β-arrestin1 activates JNK signaling pathway by binding to Src to promote the cell proliferation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Supervivencia Celular
/
Arrestinas
/
Células K562
/
Sistema de Señalización de MAP Quinasas
/
ARN Interferente Pequeño
/
Proliferación Celular
/
Beta-Arrestinas
/
Metabolismo
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2015
Tipo del documento:
Artículo
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