Your browser doesn't support javascript.
loading
β-arrestin1 promotes chronic myeloid leukemia cell proliferation by activating JNK signaling pathway / 南方医科大学学报
Journal of Southern Medical University ; (12): 677-681, 2015.
Artículo en Chino | WPRIM | ID: wpr-355305
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the signaling pathways involved in β-arrestin1-induced proliferation of K562 cells.</p><p><b>METHODS</b>We established stable cell lines K562-siβ1 and K562-β1 by lentivirus-mediated β-arrestin1 knock-down or overexpression in K562 cells, with cells transfected with non-specific siRNA as the control (K562-Ctrl). The proliferation of these cells were evaluated by cell counting and CCK-8 assays. Western blotting was used to detect the expression of JNK and p-JNK in the cells, and co-immunoprecipitation (Co-IP) assay was employed to investigate the interaction between β-arrestin1 and Src.</p><p><b>RESULTS</b>K562-β1 cells showed significantly greater but K562-siβ1 cells had significantly lower proliferation ability and cell survival rate than K562-Ctrl cells. Western blotting showed that β-arrestin1 specifically enhanced the expression of p-JNK, and the JNK inhibitor SP600125 obviously suppressed p-JNK and cell proliferation of K562 cells. Co-IP assay revealed the binding of β-arrestin1 to Src.</p><p><b>CONCLUSIONS</b>In K562 cells, β-arrestin1 activates JNK signaling pathway by binding to Src to promote the cell proliferation.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Leucemia Mielógena Crónica BCR-ABL Positiva / Supervivencia Celular / Arrestinas / Células K562 / Sistema de Señalización de MAP Quinasas / ARN Interferente Pequeño / Proliferación Celular / Beta-Arrestinas / Metabolismo Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Leucemia Mielógena Crónica BCR-ABL Positiva / Supervivencia Celular / Arrestinas / Células K562 / Sistema de Señalización de MAP Quinasas / ARN Interferente Pequeño / Proliferación Celular / Beta-Arrestinas / Metabolismo Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2015 Tipo del documento: Artículo