Regulatory T cells in the treatment of autoimmune myositis in mice: efficacy and mechanism / 南方医科大学学报
Journal of Southern Medical University
;
(12): 602-605, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-355318
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effect of CD4(+) CD25(+) Foxp3(+) Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms.</p><p><b>METHODS</b>Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4(+) CD25(+) Foxp3(+) Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs was analyzed using flow cytometry; peripheral blood IL-10 and TGF-β levels were tested using double antibody sandwich ELISA.</p><p><b>RESULTS</b>Compare with the model group, the mice in the treatment group showed significantly reduced muscular inflammatory cell infiltration, increased blood levels of IL-10 and TGF-β (P<0.05), and increased expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs (P<0.05).</p><p><b>CONCLUSION</b>CD4(+) CD25(+) Foxp3(+) Tregs reinfusion produces therapeutic effect in mice with EAM by increasing peripheral blood IL-10 and TGF-β levels and PD-1 and CTLA-4 expressions in spleen CD4(+) CD25(+) Foxp3(+) Tregs.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedades Autoinmunes
/
Bazo
/
Sangre
/
Separación Celular
/
Interleucina-10
/
Linfocitos T Reguladores
/
Modelos Animales de Enfermedad
/
Alergia e Inmunología
/
Factor de Crecimiento Transformador beta1
/
Antígeno CTLA-4
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2015
Tipo del documento:
Artículo
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