The T-lymphocyte clonal proliferation in a patient with chronic eosinophilic leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 405-408, 2003.
Article
en Zh
| WPRIM
| ID: wpr-355636
Biblioteca responsable:
WPRO
ABSTRACT
The gene fingerprinting of T cell receptor beta varial region (TCRbetaV) was used to analyze the T cell clone variations in peripheral blood lymphocytes from a patient with chronic eosinophilic leukemia (CEL) and to find out the characters of CDR3 amino acid sequence related to CEL. RT-PCR was used to amplify betaV1-24 family genes and the gene fingerprinting was set up by denatured polyacrylamide sequence gel electrophoresis. The expanded band was cut and then sequenced. The results showed that one clonal expansion of T cells could be found in TCRbetaV13.2 family. Its nucleotide sequence indicated a monoclonality. The CDR3 amino acid sequence was SFSYEQY and the modif SFSY was special reserved sequence. The other TCRbetaV families remained unchanged. It is concluded that the monoclonal T cells in TCRbetaV13.2 may be related to CEL and it is conjectured that the monoclonal T cells proliferation is induced by CEL malignant cells.
Texto completo:
1
Índice:
WPRIM
Asunto principal:
Datos de Secuencia Molecular
/
Activación de Linfocitos
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Linfocitos T
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Química
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Enfermedad Crónica
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Secuencia de Aminoácidos
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Síndrome Hipereosinofílico
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Genes Codificadores de la Cadena beta de los Receptores de Linfocito T
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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Regiones Determinantes de Complementariedad
Límite:
Aged
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Humans
/
Male
Idioma:
Zh
Revista:
Journal of Experimental Hematology
Año:
2003
Tipo del documento:
Article