Detection of minimal residual disease in Ph+/bcr-abl+ acute lymphoblast leukemia by cytogenetic analysis, nested-PCR and flow cytometry / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 372-375, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-355643
ABSTRACT
To explore a simple and sensitive method to detect minimal residual disease (MRD) in Ph(+)/bcr-abl(+) ALL patients, the bone marrow samples from 84 de novo ALL patients were detected by cytogenetic analysis, nested-PCR and flow cytometry (FCM). Cytogenetic analysis method is used to detect Ph chromosome, nested-PCR and FCM are used to detect bcr/abl mRNA and an abnormal B-cell differentiation pattern in de novo and complete remission (CR) patients, respectively. The results showed that Ph chromosome has not been found in 14 cases of CR; bcr/abl fusion gene was detected in 11 of 14 CR patients by nested-PCR (78.57%) and bcr/abl fusion gene was positive in 5 of 14 in CR patients (35.71%) by FCM. The sensitivity of nested-PCR was 10(-6)-10(-7), and that of FCM was 10(-4)- 10(-5). It is concluded that the cytogenetic analysis is not sensitive for MRD detection, and the sensitivity of nested-PCR is higher than that of FCM in detecting MRD.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Recurrencia
/
Cromosoma Filadelfia
/
Proteínas de Fusión bcr-abl
/
Sensibilidad y Especificidad
/
Neoplasia Residual
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Diagnóstico
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
/
Citometría de Flujo
/
Genética
Tipo de estudio:
Estudio diagnóstico
Límite:
Adolescente
/
Adulto
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2003
Tipo del documento:
Artículo
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