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The enhancing effects of IL-3 gene transfected murine bone marrow stromal cells on hematopoiesis under control of doxycycline / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 335-340, 2003.
Artículo en Chino | WPRIM | ID: wpr-355651
ABSTRACT
To study enhancing effect of IL-3 gene transfected bone marrow stromal cell which can be induced by doxycycline (Dox) to express IL-3 cytokine on the proliferation and differentiation of hematopoietic stem cell, retrovirus vector system contained mIL-3 cDNA was established and bone marrow stromal cell line was transfected, and obtained QXMSC1Tet-on-IL-3, in which expression level of IL-3 can be modulated by Dox. The activities of IL-3 were measured under different Dox concentrations. The numbers of hematopoietic progenitors (CFU-GM, CFU-E, CFU-GEMM and LTC-IC) were measured and the capacity of QXMSC1Tet-on-IL-3 sustaining hematopoietic progenitor cell growth was evaluated. The results showed that IL-3 gene transfected stromal cell line QXMSC1-Tet-on + IL-3 expressed high concentration of IL-3 in vitro under control of Dox. The supernatant of QXMSC1-Tet-on + IL-3 was able to increase the number of CFU-GM, CFU-E and CFU-GEMM. The total numbers of nucleated cells and long-term cultured colonies increased in LTC-IC assay. It is concluded that in the culture of QXMSC1-Tet-on + IL-3 cells, Dox actually enhanced IL-3 expression, and thus augmented the proliferation and differentiation of hematopoietic stem/progenitor cells in vitro.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fisiología / Células de la Médula Ósea / Células Madre Hematopoyéticas / Transfección / Diferenciación Celular / Interleucina-3 / Células del Estroma / Doxiciclina / Biología Celular Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2003 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fisiología / Células de la Médula Ósea / Células Madre Hematopoyéticas / Transfección / Diferenciación Celular / Interleucina-3 / Células del Estroma / Doxiciclina / Biología Celular Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2003 Tipo del documento: Artículo