Effect of angiotensin II on cord blood CD34(+) cells expansion in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 227-229, 2003.
Artículo
en Chino
| WPRIM
| ID: wpr-355677
ABSTRACT
Angiotensin II (Ang II) is one active substance of renin-angiotensin system. In order to explore the effect of Ang II combined with various cytokines on proliferation and differentiation of cord blood CD34(+) cells, in vitro experiments of cell cultures of Ang II with or without cytokines were taken place. The results showed that Ang II stimulated both BFU-E and CFU-GM expansion. The numbers of BFU-E and CFU-GM raised with increase of Ang II concentrations ranged from 0.01 - 0.1 micro mol/L. In semi-solid culture assay, Ang II stimulated CFU-GM production but no effect on BFU-E occurred. The multiple number of CFU-GM increased from 2.3 +/- 0.8 to 7.8 +/- 1.9 times when Ang II was added into SCF + G-CSF + GM-CSF + IL-3 combination. Similarly, the multiple number of BFU-E increased from 3.1 +/- 1.8 to 9.2 +/- 2.3 times when Ang II was combined with SCF + EPO + TPO + IL-3. In conclusion, Ang II stimulated cord blood hematopoietic stem/progenitor cell expansion in vitro the in presence of various cytokines.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Trombopoyetina
/
Sangre
/
Angiotensina II
/
Células Madre Hematopoyéticas
/
Células Precursoras Eritroides
/
Diferenciación Celular
/
División Celular
/
Células Cultivadas
/
Factor Estimulante de Colonias de Granulocitos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2003
Tipo del documento:
Artículo
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