Comprehensive chemical pattern recognition of pantagirus semen / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a method of comprehensive chemical pattern recognition of plantain seed via HPLC fingerprint, principal component analysis (PCA) and cluster analysis.</p><p><b>METHOD</b>The chromatographic separation was performed on a C18 column (4.6 mm x 250 mm, 5 microm). The mobile phase was a mixture of acetonitrile-0.5% acetic acid aqueous solution in gradient elution. The HPLC fingerprint of ethyl acetate fraction of 24 batches Plantaginis Semen from different habits and varieties was set up and 10 common peaks were obtained.</p><p><b>RESULT</b>The result of the principal component analysis (PCA) and cluster analysis is similar but there is disparity between them.</p><p><b>CONCLUSION</b>The method could be used for the quality control and comprehensive evaluation of Plantaginis Semen.</p>