Cloning and sequence analysis of squalene synthase gene and cDNA in Glycyrrhiza uralensis / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1416-1420, 2011.
Artículo
en Chino
| WPRIM
| ID: wpr-356107
ABSTRACT
<p><b>OBJECTIVE</b>To clone and sequence the open reading frame and genomic sequence of squalene synthase (SQS) from Glycyrrhiza uralensis.</p><p><b>METHOD</b>The primers were designed according to cDNA sequence of SQS from G. glabra reported by Hiroaki HAYASHI, SQS cDNA was cloned with total RNA extracted from roots of G. uralensis. Specific fragments were amplified by RT-PCR and then were cloned and sequenced. SQS DNA was cloned with total DNA extracted from roots of G. uralensis. Specific fragments were amplified by PCR and then were cloned and sequenced.</p><p><b>RESULT</b>GuSQS1 (GenBank accession number GQ266154) was 1 242 bp in length encoding proteins with 412 amino acid. NCBI Blast x search results showed GuSQS1 had the highest amino acid similarity to the corresponding proteins from G. uralensis. The identities of GuSQS1 with the two proteins were 98. 55% and 88. 62%. SQS (GenBank accession number GQ180932) gene with 4 484 bp containing 13 exons and 12 introns was then amplified by PCR with genomic DNA extracted from roots of G. uralensis.</p><p><b>CONCLUSION</b>These findings of cloning and sequencing the open reading frame and genomic sequence of squalene synthase (SQS) from G. uralensis brought some new clues for the further exploration of SmSQS function in sterol and terpenes biosynthesis.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farnesil Difosfato Farnesil Transferasa
/
Datos de Secuencia Molecular
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Química
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Sistemas de Lectura Abierta
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Secuencia de Aminoácidos
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Clonación Molecular
/
Análisis de Secuencia de ADN
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ADN Complementario
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Raíces de Plantas
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Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2011
Tipo del documento:
Artículo
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