Roles of CD8+ CD28- T regulatory cells in acute infectious mononucleosis in children / 中华儿科杂志
Chinese Journal of Pediatrics
; (12): 208-211, 2007.
Article
en Zh
| WPRIM
| ID: wpr-356200
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of CD(8)(+)CD(28)(-) T regulatory cells (Tr) in the immunological pathogenesis of acute infection with Epstein-Barr virus in children.</p><p><b>METHODS</b>The present study enrolled 25 children with infectious mononucleosis (IM) and 25 age-matched healthy children. Flow cytometric analysis was performed to detect the percentage of CD(3)(+), CD(3)(+)CD(4)(+), CD(3)(+)CD(8)(+), CD(8)(+)CD(28)(+) by determining the ratio of positive cells in lymphocytes. Reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time PCR were used to analyze IL-6, IL-10, IFN-gamma expression in CD(8)(+)CD(28)(-) Tr cells and ILT-3, ILT-4 expression in monocytes/macrophages.</p><p><b>RESULTS</b>The proportions of CD(8)(+)CD(28)(-)T cells in children with acute-phase IM was significantly higher than those in the controls (P < 0.01). The expression level of IL-6, IL-10, IFN-gamma, ILT-3, ILT-4 mRNA significantly increased compared to those of the controls (P < 0.01).</p><p><b>CONCLUSION</b>The CD(28) expressed on CD(8)(+) T cells in vivo is gradually lost with age and CD(8)(+)CD(28)(-) cells increase up 50% to adult. EBV can directly infect B cells, trigger CD(8)(+) CTL response and destroy the target cells to cause serious immunopathological lesion. Therefore we speculate that the expansion of CD(8)(+)CD(28)(-) Tr cells in children with IM may be an adaptive immune response to avoid serious inflammation and autoimmune reactions.</p>
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Índice:
WPRIM
Asunto principal:
Virología
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Glicoproteínas de Membrana
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Receptores Inmunológicos
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Estudios de Casos y Controles
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Citocinas
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Linfocitos T Reguladores
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Antígenos CD28
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Herpesvirus Humano 4
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Receptores de Superficie Celular
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Linfocitos T CD8-positivos
Tipo de estudio:
Observational_studies
Límite:
Child
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Child, preschool
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Pediatrics
Año:
2007
Tipo del documento:
Article