Analysis of sequence-tagged site in bcr and abl genes by DNA pooling and dHPLC / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 468-471, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-356535
ABSTRACT
To investigate the relationship between the single nucleotide polymorphism (SNPs) of the bcr and abl gene and chronic myelogeous leukemia (CML), the 9 sequence-tagged sites (STS) in bcr and abl gene were screened by DNA pooling and denaturing high performance liquid chromatography (dHPLC), and the results were varified by sequencing. The results showed that the polymorphism sites were detected in 4 out of the 9 STS fragments and there were 3 bases different from the reference sequence found in 3 fragments. In conclusion, the novel SNP in U07000 fragment shows significantly different frequencies between CML and controled people.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Leucemia Mielógena Crónica BCR-ABL Positiva
/
Genes abl
/
Cromatografía Líquida de Alta Presión
/
Lugares Marcados de Secuencia
/
Proteínas de Fusión bcr-abl
/
Análisis de Secuencia de ADN
/
Polimorfismo de Nucleótido Simple
/
Proteínas Proto-Oncogénicas c-bcr
/
Genética
/
Métodos
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
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