Preparation of transfusable human universal red blood cell with recombinant alpha-galactosidase / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 313-316, 2005.
Artículo
en Chino
| WPRIM
| ID: wpr-356570
ABSTRACT
In order to meet the demand for safe transfusion in special conditions and to utilize the donated blood supply efficiently, technology has been developed to convert erythrocytes from type A, B, or AB to "universal donor" blood. Conversion of blood type B to O was performed by means of recombinant alpha-galactosidase digestion. The results showed that blood type B to O was converted successfully, 1 transfusion unit of red cells of group B (100 ml totally) could converted to universal blood cells in the optimal conditions including pH 5.6, 26 degrees C, 2 hours, obturation and sterilization. It is concluded that the universal red blood cells converted from group B to group O are conformed to demand of identification rules of biological products, no harmful effects of alpha-galactosidase on cell structure and function are observed. The converted red cells can stored in 4 degrees C for 21 days.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Incompatibilidad de Grupos Sanguíneos
/
Transfusión Sanguínea
/
Sistema del Grupo Sanguíneo ABO
/
Proteínas Recombinantes
/
Ensayo de Inmunoadsorción Enzimática
/
Clasificación
/
Café
/
Alfa-Galactosidasa
/
Electroforesis en Gel de Poliacrilamida
Tipo de estudio:
Estudio pronóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Experimental Hematology
Año:
2005
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS