Your browser doesn't support javascript.
loading
Effect of bone marrow stromal cells on the apoptotic sensitivity of HL-60 and HL-60/VCR cells / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 286-292, 2005.
Artículo en Chino | WPRIM | ID: wpr-356575
ABSTRACT
This study was aimed to investigate the effects of human bone marrow fibroblastoid stromal cell line (HFCL) on chemosensitivity of acute myeloid leukemia sensitive HL-60 cell line and multidrug-resistant (MDR) HL-60/VCR cell line in vitro co-culture. Setting up co-culture system of HL-60 or HL-60/VCR cells in direct contact with HFCL cells, or with HFCL cells separated by transwell, and exposing HL-60 or HL-60/VCR cells to different concentrations of topotecon (TPT), morphologic evidence for apoptosis was determined by staining with Wright-Giemsa stain and acridine orange/ethidium bromide (AO/EB). Cell cycle, sub-G(1) and annexin V FITC staining were detected by flow cytometry. The expression of active caspase-3, Bcl-2 and Pgp was detected by Western blot. The results showed that HL-60 or HL-60/VCR cells treated by TPT revealed characteristic apoptotic morphological changes by Wright-Giemsa and AO/EB staining. The percentage of annexin V-positive cells and apoptotic cells decreased when they were cocultured with HFCL cells. The proportion of G(0)/G(1) HL-60 or HL-60/VCR cells treated by TPT increased and the sub-G(1) appeared significantly, but apoptotic and sub-G cells reduced after direct contact with HFCL cells. Meanwhile, although HL-60 or HL-60/VCR cells treated by TPT expressed activated caspase-3, and the expression of Bcl-2 decreased, the expression of activated caspase-3 decreased and Bcl-2 increased after direct contact with HFCL cells. In conclusion, HFCL stromal cells can prevent TPT-induced apoptosis in HL-60 and HL-60/VCR cells via modulation of Bcl-2 and active caspase-3.
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fisiología / Vincristina / Células de la Médula Ósea / Ciclo Celular / Línea Celular / Western Blotting / Células del Estroma / Apoptosis / Células HL-60 Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Farmacología / Fisiología / Vincristina / Células de la Médula Ósea / Ciclo Celular / Línea Celular / Western Blotting / Células del Estroma / Apoptosis / Células HL-60 Tipo de estudio: Estudio diagnóstico Límite: Humanos Idioma: Chino Revista: Journal of Experimental Hematology Año: 2005 Tipo del documento: Artículo