Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein / 病毒学报
Chinese Journal of Virology
;
(6): 253-256, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-356607
ABSTRACT
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 112 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Rabia
/
Virus de la Rabia
/
Virología
/
Datos de Secuencia Molecular
/
Química
/
Secuencia de Aminoácidos
/
Mapeo Epitopo
/
Epítopos de Linfocito B
/
Alergia e Inmunología
/
Genética
Límite:
Animales
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2014
Tipo del documento:
Artículo
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