IL15 DNA adjuvant enhances cellular and humoral immune responses induced by DNA and adenoviral vectors encoding HIV-1 subtype B gp160 gene / 病毒学报
Chinese Journal of Virology
;
(6): 62-65, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-356637
ABSTRACT
To enhance the immunogenicity of DNA and adenoviral vector vaccines expressing HIV-1 subtype B gp160, human interleukin 15 (hIL15) DNA adjuvant (pVR-hIL15) was constructed. BALB/c mice received DNA prime/protein boost immunization with pVR-HIVgp160/Ad5-HIVgp160 alone or combined with pVR-hIL15. Cellular and humoral immune responses were evaluated by IFN-gamma enzyme-linked immunosorbent spot assay and enzyme-linked immunosorbent assay, respectively. Compared with those immunized with vaccines alone, the mice immunized with vaccines combined with pVR-hIL15 had significantly increased specific cellular response and antibody titer (P < 0.05). It suggests that the IL15 DNA adjuvant can enhance the immune responses induced by prime-boost regimen using DNA and adenoviral vector encoding HIV-1 subtype B gp160.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteína gp41 de Envoltorio del VIH
/
Proteína gp120 de Envoltorio del VIH
/
Adenoviridae
/
Adyuvantes Inmunológicos
/
Vacunas de ADN
/
Proteínas gp160 de Envoltorio del VIH
/
Interleucina-15
/
Alergia e Inmunología
/
Inmunidad Humoral
/
Vectores Genéticos
Límite:
Animales
/
Femenino
/
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2014
Tipo del documento:
Artículo
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