HIV-1 infection up-regulating expression of interferon-stimulated gene 15 in cell lines / 病毒学报
Chinese Journal of Virology
;
(6): 480-487, 2013.
Artículo
en Chino
| WPRIM
| ID: wpr-356678
ABSTRACT
To investigate whether HIV-1 infection affects expression of interferon-stimulated gene 15 (ISG15) and determine the antiviral effect of ISG15 in vitro, ISG15 expression at transcription and protein level and supernatant p24 of HIV-1 was detected in various HIV-1 infected or transfected cell lines, respec tively. HIV-1 molecular clone pNL4-3 was used to transfect 293T, TZM-bl and HeLa cells while HIV-1 pseudo-typed virus was used to infect Jurkat, MT-1 and THP-1 cells. After twenty-four hours post infection or transfection, cells were harvested for extraction of total RNAs and subsequently used in real time PCR for quantification of ISG15 transcriptional expression. After forty-eight hours post infection or transfection, cells were harvested for extraction of total proteins to detect ISG15 protein expression. A significant up-regulation of ISG15 at transcription level was observed in HIV-1 infected or transfected cell lines, particulaly in THP-1 and TZM-bl cells. Up-regulation of ISG15 protein was observed only in TZM-bl cell. Cotransfection of ISG15 and HIV-1 indicated that ISG15 inhibited production of HIV-1 progeny virus in a dose and time depend manner in 293T cell but not TZM-bl cell. These results revealed upregulating ISG15 expression in transcriptional level and potential antagonistic mechanism against ISG15 by HIV-1 infection, simultanelusly.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Virología
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Ubiquitinas
/
Infecciones por VIH
/
Regulación hacia Arriba
/
Línea Celular
/
Citocinas
/
VIH-1
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2013
Tipo del documento:
Artículo
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