Studies on HPLC fingerprint of Radix Aconiti Kusnezoffii / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish the HPLC fingerprint of Radix Aconiti Kusnezoffii.</p><p><b>METHOD</b>The chromatographic separation was performed on a Kromasil C18 (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of acetonitrile-ammonium acetate buffer (2. 5 per thousand acetic acid-ammonia pH 10.5) (6040). The UV detection wavelength was set at 240 nm and the flow rate was set at 1.0 mL x min(-1).</p><p><b>RESULT</b>The RSD of precision and repeatability was less than 2%. Under the selected chromatographic conditions, good HPLC fingerprints of Radix Aconiti Kusnezoffii were obtained.</p><p><b>CONCLUSION</b>The method was simple, accurate and repeatable. It can be used for the quality control of Radix Aconiti Kusnezoffii.</p>