High specific PCR identification of Bungarus multicinctus and its adulterants / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1050-1053, 2006.
Artículo
en Chino
| WPRIM
| ID: wpr-356704
ABSTRACT
<p><b>OBJECTIVE</b>To develop a convenient and effective method for the identification of Bungarus multicinctus.</p><p><b>METHOD</b>Based on the sequence of Cyt b gene fragment of B. multicinctus and its adulterants, a pair of highly specific primer (HJL- and HJH-) were designed for distinguishing B. ulticinctus from other species of snake. To establish specific PCR reaction condition, the primers were employed to amplify the DNA templates extracted from B. multicinctus and 6 other species of snake, under different annealing temperature. Using this method, B. multicinctus was identified from 18 samples bought from many drugstores.</p><p><b>RESULT</b>A 230 bp DNA fragment was amplified from B. multicinctus in PCR with annealed temperature at 67 degrees C, whereas no DNA fragment was amplified from other snake samples under the same reaction condition, B. multicinctus could be clearly distinguished from others by PCR reaction with the highly specific primers. In the present study, 18 sample, bought from different drugstores, were also identified by the highly specific PCR with the primers. The results indicated that 14 samples were B. multicinctus and the other 4 were adulterant, which was consistent with the conclusion of authentication based on morphological.</p><p><b>CONCLUSION</b>The primers designed in the present study were highly specific for B. multicinctus.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Serpientes
/
Especificidad de la Especie
/
ADN
/
Materia Medica
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Reacción en Cadena de la Polimerasa
/
Contaminación de Medicamentos
/
Clasificación
/
Análisis de Secuencia de ADN
Tipo de estudio:
Estudio diagnóstico
Límite:
Animales
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2006
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS