Rhein inhibits transforming growth factor beta1 induced plasminogen activator inhibitor-1 in endothelial cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 354-359, 2003.
Article
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| WPRIM
| ID: wpr-356798
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WPRO
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the effect of rhein on endothelial plasminogen activator inhibitor-1 (PAI-1) mRNA expression and protein production induced by transforming growth factor beta1 (TGFbeta1), and to explore the mechanism of the protective action of rhein on endothelial cells.</p><p><b>METHODS</b>A human umbilical endothelium derived cell line (ECV-304) from ATCC was used in this study. The PAI-1 mRNA expression and protein synthesis in the endothelial cells were detected by Northern blot and flow cytometry analysis, respectively. The activity of phospho-p44/p42 MAP kinase induced by TGFbeta1 was determined by immunoprecipitation analysis and western blot.</p><p><b>RESULTS</b>TGFbeta1 rapidly increased PAI-1 mRNA expression in the endothelial cells, and this effect lasted at least 24 hours. The upregulation of PAI-1 mRNA expression induced by TGFbeta1 in endothelial cells was inhibited by rhein in a dose-dependent manner. In addition, rhein inhibited endothelial PAI-1 protein production. Further study revealed that rhein had a significant inhibitory effect on the activity of phospho-p44/p42 MAP kinase induced by TGFbeta1 in human endothelial cells.</p><p><b>CONCLUSIONS</b>Our results showed that rhein may have a protective effect on the endothelial dysfunction by inhibiting overexpression of PAI-1, indicating a way for the treatment of vascular diseases.</p>
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Asunto principal:
Farmacología
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ARN Mensajero
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Endotelio Vascular
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Células Cultivadas
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Factor de Crecimiento Transformador beta
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Antraquinonas
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Inhibidor 1 de Activador Plasminogénico
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Proteína Quinasa 1 Activada por Mitógenos
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Proteínas Quinasas Activadas por Mitógenos
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Proteína Quinasa 3 Activada por Mitógenos
Límite:
Humans
Idioma:
En
Revista:
Chinese Medical Journal
Año:
2003
Tipo del documento:
Article