Changes in gap junctional intercellular communication in rabbits lens epithelial cells induced by low power density microwave radiation / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1873-1876, 2002.
Artículo
en Inglés
| WPRIM
| ID: wpr-356889
ABSTRACT
<p><b>OBJECTIVE</b>To demonstrate the changes in gap junctional intercellular communication (GJIC) mediated by low power density microwave radiation in rabbits lens epithelial cells (LECs) and its mechanisms.</p><p><b>METHODS</b>Rabbits' eyes were exposed to 5 mW/cm(2) and 10 mW/cm(2) power densities of microwave radiation for 3 hours. The fluorescence-recovery-after-photobleaching (FRAP) method was used to determine the GJIC. The localization and function of connexin 43 in LECs was detected by laser scanning confocal microscopy.</p><p><b>RESULTS</b>The GJIC of rabbits LECs was inhibited by microwave radiation especially in the 10 mW/cm(2) irradiated samples. A decrease in connexin 43-positive staining was seen in 5 mW/cm(2) x 3 h treated LECs. Intracellular space accumulation and cytoplasmic internalization were clearly demonstrated in 10 mW/cm(2) group.</p><p><b>CONCLUSIONS</b>Low power densities microwave radiation (5 mW/cm(2) and 10 mW/cm(2)) induces damage to connexin 43 and inhibits the GJIC of rabbits LECs. These changes result in an osmotic imbalance within the lens and induce early cataract. 5 mW/cm(2) or 10 mW/cm(2) microwave radiation is cataractogenic.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Efectos de la Radiación
/
Catarata
/
Comunicación Celular
/
Uniones Comunicantes
/
Conexina 43
/
Técnica del Anticuerpo Fluorescente Indirecta
/
Células Epiteliales
/
Cristalino
/
Microondas
Límite:
Animales
Idioma:
Inglés
Revista:
Chinese Medical Journal
Año:
2002
Tipo del documento:
Artículo
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