Changes of hypoxia-inducible factor in residual hepatocellular carcinoma following high-intensity focused ultrasound exposure in nude mice / 南方医科大学学报
Journal of Southern Medical University
;
(12): 463-467, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-356898
ABSTRACT
<p><b>OBJECTIVE</b>To study the changes in hypoxia-inducible factor (HIF1α, HIF2α) in the residual tumor cells in nude mice bearing hepatocellular carcinoma (HCC) following treatment with high-intensity focused ultrasound (HIFU).</p><p><b>METHODS</b>Thirty nude mice bearing human HCC received treatment with HIFU. At 1, 3, and 5 days and 1 and 2 weeks after the treatment, the mice were examined for pathological changes of the residual tumor with HE staining; SP immunohistochemistry, Western blotting and real-time quantitative PCR were used to detect the protein and mRNA expressions of HIF1α and HIF2α in the tumor.</p><p><b>RESULTS</b>HE staining revealed the presence of residual tumor cells and large necrotic areas after the treatment. Immunohistochemistry showed a gradual increment of HIF1α protein and mRNA expressions after the treatment, reaching the peak level at 3 days (P<0.05) followed by progressive reduction at 5 days and 1 and 2 weeks. HIF2α expressions at either the protein or mRNA levels exhibited no significant changes within 3 days after the treatment (P>0.05) but increased significantly at 5 days and 1 and 2 weeks (P<0.05).</p><p><b>CONCLUSION</b>The changes of HIF1α and HIF2α in the residual tumor after HIFU treatment in nude mice bearing HCC can be associated with tumor cell apoptosis and angiogenesis after the treatment.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
Terapéutica
/
Terapia por Ultrasonido
/
Carcinoma Hepatocelular
/
Neoplasia Residual
/
Subunidad alfa del Factor 1 Inducible por Hipoxia
/
Células Hep G2
/
Neoplasias Hepáticas
/
Metabolismo
/
Métodos
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2014
Tipo del documento:
Artículo
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