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Construction of pcDNA3-HERG-G572R expression vector and establishment of a cell line stably expressing HKE-HERG-G572R / 南方医科大学学报
Journal of Southern Medical University ; (12): 308-311, 2014.
Artículo en Chino | WPRIM | ID: wpr-356931
ABSTRACT
<p><b>OBJECTIVE</b>To construct the pcDNA3-HERG-G572R expression vector and establish a cell line stably expressing HKE-HERG-G572R.</p><p><b>METHODS</b>HERG-G572R mutant fragment was constructed by over-lap extension PCR and validated by DNA sequencing. The HKE-HERG-G572R expression vector was constructed and transfected into HEK293 cells to obtain a cell line stably expressing HKE-HERG-G572R.</p><p><b>RESULTS</b>The pcDNA3-HERG-G572R expression vector was successfully constructed and the cell line stably expressing HKE-HERG-G572R was established. Real-time PCR and Western blotting revealed a 632-fold HKE-HERG-G572R overexpression in the transfected HEK293 cells as compared with that in control HEK293 cells transfected with pcDNA3 (P<0.01).</p><p><b>CONCLUSION</b>The protocol can be used to construct the cell line stably expressing HKE-HERG-G572R to provide a cell model for studying individualized therapy.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Secuencia de Bases / Transfección / Expresión Génica / Canales de Potasio Éter-A-Go-Go / Células HEK293 / Vectores Genéticos / Genética / Metabolismo / Mutación Tipo de estudio: Guía de Práctica Clínica Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2014 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Secuencia de Bases / Transfección / Expresión Génica / Canales de Potasio Éter-A-Go-Go / Células HEK293 / Vectores Genéticos / Genética / Metabolismo / Mutación Tipo de estudio: Guía de Práctica Clínica Límite: Humanos Idioma: Chino Revista: Journal of Southern Medical University Año: 2014 Tipo del documento: Artículo