Effects of farnesoid X receptor agonist on adiponectin and its receptors / 南方医科大学学报
Journal of Southern Medical University
;
(12): 109-112, 2014.
Artículo
en Chino
| WPRIM
| ID: wpr-356975
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of GW4064, a farnesoid X receptor (FXR) agonist, on adiponectin and its receptors during the differentiation of 3T3-L1 preadipocytes and on adiponectin receptors in HepG2 cells.</p><p><b>METHODS</b>The mRNA expressions of FXR, PPARγ2, adiponectin, AdipoR1, and AdipoR2 and the protein levels of adiponectin on days 0, 2, 4, 6, and 8 during the differentiation of 3T3-L1 preadipocytes treated with GW4064 were detected by fluorescent real-time PCR and ELISA, respectively. The mRNA expressions of AdipoR1 and AdipoR2 in HepG2 cells were also examined at 0, 12, 24, and 48 h after GW4064 treatment.</p><p><b>RESULTS</b>The mRNA expressions of FXR, PPARγ2, adiponectin, and AdipoR2 in 3T3-L1 preadipocytes and AdipoR2 in HepG2 cells treated with GW4064 was significantly increased compared with the control group (all P<0.05). The protein level of adiponectin was also significantly increased after GW4064 treatment. The expression of AdipoR1 in either 3T3-L1 preadipocytes or HepG2 cells showed no significant changes after GW4064 treatment.</p><p><b>CONCLUSION</b>GW4064 can up-regulate the expressions of FXR, PPARγ2, adiponectin, AdipoR2 in 3T3-L1 preadipocytes and AdipoR2 in HepG2 cells. As adiponectin and its receptors are two important factors in the treatment of non-alcoholic fatty liver disease, FXR agonist may potentially produce therapeutic effect on non-alcoholic fatty liver disease and can regulate adipocytes via up-regulating PPARγ during adipocyte differentiation.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Farmacología
/
Diferenciación Celular
/
Receptores Citoplasmáticos y Nucleares
/
Células 3T3-L1
/
PPAR gamma
/
Adiponectina
/
Receptores de Adiponectina
/
Células Hep G2
/
Isoxazoles
/
Metabolismo
Límite:
Animales
/
Humanos
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2014
Tipo del documento:
Artículo
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