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Follicular Helper T Cells and Expression of PD-1 in Mice with Myelodysplastic Syndrome / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 756-760, 2015.
Artículo en Chino | WPRIM | ID: wpr-357276
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the complete blood count, morphological changes, follicular T helper (Tfh) cells and expression of PD-1 in bone marrow and spleen of mice with myelodysplastic syndrome(MDS) and to explore their significance in pathogenesis of MDS.</p><p><b>METHODS</b>The 10 male NUP98-HOXD13 transgenic mice and 10 male homologous wild-type C57BL/6J mice were used for experments. The complete blood count, morphological change of NUP98-HOXD13 transgenic mice and wild-type C57BL/6J were detected by routine methods. The level of Tfh cells and expression of PD-1 in bone marrow and spleen were measured by flow cytometry. The PD-1 mRNA of bone marrow mononuclear cells and spleen cells were analyzed by real-time PCR method.</p><p><b>RESULTS</b>The counts of RBC, neutrophile and platelet in above- mentioned transgenic mice were less than that in wild type C57BL/6J mice. As compared with wild type C57BL/6J mice, the morphology of RBC and platelet in transgenic mice was some abnormal, including bi-nucleated erythrocytes, ringed mucleated neutrophil and erythroblastic islands. The count of Tfh cells in transgenic mice was less than that in wild type mice, but the expression of PD-1 was higher. The expression of BMMNC PD-1 mRNA was obviously higher than that in wild type mice.</p><p><b>CONCLUSION</b>The pancytopenia and dysplasia, decrease of Tfh cells and increase of PD-1 expression have been observed in NUP98-HOXD13 transgenic mice, which may be one of important reasons for promoting malignant clone and leading to impair anti immune respones.</p>
Asunto(s)
Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pancitopenia / Médula Ósea / Síndromes Mielodisplásicos / Células de la Médula Ósea / Ratones Transgénicos / Células Cultivadas / Linfocitos T Colaboradores-Inductores / Receptor de Muerte Celular Programada 1 / Reacción en Cadena en Tiempo Real de la Polimerasa / Citometría de Flujo Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2015 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Pancitopenia / Médula Ósea / Síndromes Mielodisplásicos / Células de la Médula Ósea / Ratones Transgénicos / Células Cultivadas / Linfocitos T Colaboradores-Inductores / Receptor de Muerte Celular Programada 1 / Reacción en Cadena en Tiempo Real de la Polimerasa / Citometría de Flujo Límite: Animales Idioma: Chino Revista: Journal of Experimental Hematology Año: 2015 Tipo del documento: Artículo