Refined mapping of heterozygosity loss on chromosome 5p15 in sporadic colorectal cancer / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 413-417, 2008.
Artículo
en Chino
| WPRIM
| ID: wpr-357410
ABSTRACT
<p><b>OBJECTIVE</b>To refine the loss of heterozygosity (LOH) on chromosome 5p15 and screen new tumor suppressor gene(s) in colorectal tumorigenesis.</p><p><b>METHODS</b>Samples of colorectal cancer and normal tissue of 83 cases were collected in this study. Sixteen polymorphic microsatellite markers were analyzed on chromosome 5 and another 6 markers on chromosome 5p15 by PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis.</p><p><b>RESULTS</b>Two distinct regions of frequent allelic deletions at D5S416 on 5p15 and D5S428-D5S410 on 5q were detected. Another 6 polymorphic microsatellite markers were applied to 5p15 and the minimal region of frequrent loss of heterozygosity was established on 5p15 spanning the D5S416 locus.</p><p><b>CONCLUSION</b>A critical and precise location of 5p deletions, 5p15.2-5p15.3, has been detected, which may contain one or more unknown tumor suppressor gene(s) related to colorectal cancer.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Cromosomas Humanos Par 5
/
Neoplasias Colorrectales
/
Reacción en Cadena de la Polimerasa
/
Mapeo Cromosómico
/
Eliminación de Gen
/
Repeticiones de Microsatélite
/
Pérdida de Heterocigocidad
/
Genética
/
Métodos
Límite:
Adulto
/
Anciano
/
Aged80
/
Femenino
/
Humanos
/
Masculino
Idioma:
Chino
Revista:
Chinese Journal of Oncology
Año:
2008
Tipo del documento:
Artículo
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