Construction of recombinant adenovirus vector carrying human TIMP-1 cDNA and its expression in vitro / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 420-424, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-357685
ABSTRACT
The full-length cDNA of hTIMP-1 was obtained from a surgical patient with HCC by the method of RT-PCR. Then it was cloned into the adenoviral shuttle plasmid pAdTrack-CMV, and subsequently cotransformed into competent BJ5183 cells with the adenoviral backbone plasmid pAdEasy-1. Thereupon, a recombinant adenoviral plasmid containing full-length cDNA of hTIMP-1 was generated by homologous recombination in E. coli. The adenoviruses (AdhTIMP-1) were packaged and amplified in adenoviral packaging cells HEK 293. Then the viral titer was checked by green fluorescent protein (GFP), and the expression of hTIMP-1 was detected by the techniques of Western blot and RT-PCR. The recombinant adenovirus vector carrying human TIMP-1 was successfully constructed and expressed in vitro and may pave the way for further application in liver gene therapy.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Proteínas Recombinantes de Fusión
/
Línea Celular
/
Adenoviridae
/
Clonación Molecular
/
ADN Complementario
/
Carcinoma Hepatocelular
/
Inhibidor Tisular de Metaloproteinasa-1
/
Matriz Extracelular
/
Vectores Genéticos
/
Genética
Límite:
Humanos
Idioma:
Chino
Revista:
Journal of Biomedical Engineering
Año:
2007
Tipo del documento:
Artículo
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