Expression of phosphorylated NMDA receptor 1 in the cerebral cortex after NMDA microinjection in vivo / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 51-53, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-357751
ABSTRACT
<p><b>OBJECTIVE</b>The activation of N-methyl-D-aspartate(NMDA) receptors plays critical roles in the pathogenesis of diseases of the brain. This study aimed to examine the expression of phosphor-NR1 S897 in the cerebral cortex after NMDA microinjection in vivo.</p><p><b>METHODS</b>Forty seven-day-old Sprague-Dawley rats were randomly assigned into normal control and NMDA injection groups. The rats from the NMDA injection group were injected with 10 mmol of NMDA and were sacrificed 1 hr after injection. 2, 3, 5-triphenyltetrazolium chloride (TTC) and fluorescent immunohistochemical stainings were conducted and the fluorescence intensity OD value between the two groups was compared.</p><p><b>RESULTS</b>TTC staining from the two groups was normal. Expression of phosphor-NR1 S897 in the cerebral cortex of the ipsilateral hemisphere to injection in the NMDA injection group decreased significantly compared with the normal control group, with OD values of 0.366 +/- 0.087 vs 1.364 +/- 0.268 (P < 0.01).</p><p><b>CONCLUSIONS</b>NMDA microinjection, as a hypoxia-ischemia (HI) insult, significantly decreased the expression of phosphor-NR1 S897. This indicates the importance of the "HI-NMDA-phospho-NR1 S897 dephosphorylation-cell damage" pathway in HI brain damage.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Fosforilación
/
Corteza Cerebral
/
N-Metilaspartato
/
Técnica del Anticuerpo Fluorescente
/
Ratas Sprague-Dawley
/
Receptores de N-Metil-D-Aspartato
/
Hipoxia-Isquemia Encefálica
/
Metabolismo
/
Microinyecciones
Límite:
Animales
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2007
Tipo del documento:
Artículo
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