Detection of MYCN mRNA in neuroblastoma cell lines by quantitative RT-PCR / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 47-50, 2007.
Artículo
en Chino
| WPRIM
| ID: wpr-357752
ABSTRACT
<p><b>OBJECTIVE</b>To examine the feasibility and practicability of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) with SYBR GREEN I fluorescence for detecting the MYCN mRNA expression in neuroblastoma cell line LA-N-5.</p><p><b>METHODS</b>MYCN mRNA expression in LA-N-5 cells was measured using real time RT-PCR with SYBR GREEN I. Glyceraldehyde phosphate dehydrogenase (GAPDH) was used as internal control. The level of the MYCN mRNA was calculated as MYCN copies/GAPDH copies.</p><p><b>RESULTS</b>Standard curves were linear and showed high correlations (R2>0.99). The ratio of MYCN mRNA copies to GAPDH mRNA copies was calculated based on specific PCR products. The MYCN mRNA level in LA-N-5 cells was obtained (17.4 +/- 1.2).</p><p><b>CONCLUSIONS</b>Quantitative RT-PCR with SYBR GREEN I fluorescence may be a sensitive and reliable method for detecting the MYCN mRNA expression. It may also be potential applicable for detecting the MYCN mRNA expression in the small amount neuroblastoma tissues.</p>
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Patología
/
ARN Mensajero
/
Proteínas Proto-Oncogénicas c-myc
/
Sensibilidad y Especificidad
/
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
/
Línea Celular Tumoral
/
Genética
/
Metabolismo
/
Métodos
/
Neuroblastoma
Tipo de estudio:
Estudio diagnóstico
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Contemporary Pediatrics
Año:
2007
Tipo del documento:
Artículo
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